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KMID : 0613820200300010045
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Journal of Life Science
2020 Volume.30 No. 1 p.45 ~ p.57
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Transcriptome Analysis of Longissimus Tissue in Fetal Growth Stages of Hanwoo (Korean Native Cattle) with Focus on Muscle Growth and Development
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Jeong Tae-Joon
Chung Ki-Yong
Park Won-Cheoul
Son Ju-Hwan
Park Jong-Eun
Chai Han-Ha
Kwon Eung-Gi
An Jun-Sang
Lee Ji-Woong
Lim Da-Jeong
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Abstract
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The prenatal period in livestock animals is crucial for meat production because net increase in the number of muscle fibers is finished before birth. However, there is no study on the growth and development mechanism of muscles in Hanwoo during this period. Therefore, to find candidate genes involved in muscle growth and development during this period in Hanwoo, mRNA expression data of longissimus in Hanwoo at 6 and 9 months post-conceptional age (MPA) were analyzed. We independently identified differentially expressed genes (DEGs) using DESeq2 and edgeR which are R software packages, and considered the overlaps of the results as final-DEGs to use in downstream analysis. The DEGs were classified into several modules using WGCNA then the modules’ functions were analyzed to identify modules which involved in myogenesis and adipogenesis. Finally, the hub genes which had the highest WGCNA module membership among the top 10% genes of the STRING network maximal clique centrality were identified. 913(6 MPA specific DEGs) and 233(9 MPA specific DEGs) DEGs were figured out, and these were classified into five and two modules, respectively. Two of the identified modules’(one was in 6, and another was in 9 MPA specific modules) functions was found to be related to myogenesis and adipogenesis. One of the hub genes belonging to the 6 MPA specific module was axin1 (AXIN1) which is known as an inhibitor of Wnt signaling pathway, another was succinate-CoA ligase ADP-forming beta subunit (SUCLA2) which is known as a crucial component of citrate cycle.
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KEYWORD
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Adipogenesis, fetal programming, Hanwoo, hub gene, myogenesis
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